Composite

Part:BBa_K2824004:Design

Designed by: Jiang Qiaochu   Group: iGEM18_NEU_China_B   (2018-10-05)


lldPRD operon promoter-GFP


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 335
    Illegal BsaI.rc site found at 994


Design Notes

The LLDPRD operon promoter extracted directly from the genome has been able to function well. The distance between O _ 1 and O _ 2 is not considered here, but the improved part can also be used in this experiment.


Source

The lldprd gene was extracted from the K12 genome. GFP gene was a commercial components which is a green fluorescent protein derived from jellyfish Aequeora victoria wild-type GFP .

References